Coding

Part:BBa_K1414000

Designed by: keisuke sugahara   Group: iGEM14_KAIT_JAPAN   (2014-10-04)


GFP+HlyA

P\This part codes GFP which have HlyA secretion tag.We use this part to secrete GFP outside E.coli. HlyA tag is secretion signal that secrete target protein via the Type I secretion pathway of gram-negative bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None